Greengate cloning

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August undefined, 2024

Weband versatile cloning system for the generation of plant transfor-mation vectors, which we named GreenGate. The GreenGate system allows rapid and efficient assembly of six … WebDec 20, 2013 · GreenGate cloning is simple and efficient since it uses only one type IIS restriction endonuclease, depends on only six types of …

GreenGate Cloning System - MGLCC

WebNational Center for Biotechnology Information WebAug 9, 2015 · The development of DNA cloning technologies and the discovery of Agrobacterium sp. as a vehicle for delivery of recombinant DNA into the chromosomes of … floor refinishing greenville sc

GreenGate cloning technology introduces enormous advantages …

WebThe plasmids used in this study were generated using either standard molecular cloning procedures (Sambrook et al., 1989) or by using the GreenGate Cloning system (Lampropoulos et al., 2013 ... WebJul 12, 2024 · GreenGate - a novel, versatile, and efficient cloning system for plant transgenesis. PLoS One 8 , e83043 (2013). Article ADS Google Scholar WebThis plasmid is designed for Golden Gate cloning using the BsaI enzyme. Plasmid Features (listed as bp in full plasmid sequence): BsaI site #1 = 2241-2251bp D-dummy = 2252-2297bp ... GreenGate - A Novel, Versatile, and Efficient Cloning System for Plant Transgenesis. Lampropoulos A, Sutikovic Z, Wenzl C, Maegele I, Lohmann JU, Forner J ... floor refinishing danbury ct

Quick, Versatile Plant Transgenesis with GreenGate Plasmids

Figure 1. The Golden Gate principle. A) Type IIS restriction...

WebThis plasmid is designed for Golden Gate cloning using the BsaI enzyme. These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were … Webday 1: amplify lvl0 unit, like promoter, terminator etc., perform GG reaction, transform; day 2: check E.coli by PCR, seed correct clones; day 3: prepare the plasmid, which can be … floor refinishing equipment rentalWebThe characteristic of GreenGate cloning technology is sufficient to simultaneously construct a variety of effector expression vectors fused with different tags. On the basis of the … great portway great denham

"WebOct 12, 2024 · The hip1 coding sequence devoid of BsaI restriction sites for GreenGate cloning was synthesized by Biocat (Germany). For recombinant protein expression, the hip1 sequence was amplified without SP and cloned into the pET28a (+) expression vector (Addgene, USA), eventually having a His-tag at the N-terminus. Sequences for cysteine … " - Greengate cloning

Greengate cloning

Comprehensive Toolkit for Inducible, Cell Type-Specific Gene …

WebGolden Gate cloning is one of the easiest cloning methods in terms of hands-on time, as digestion and ligation can be done in one 30-minute reaction. The destination vector and entry vector(s) are placed in a … Webgreen gate cloning 중인데 cloning이 처음입니다 mod c인 cds를 만들던 중 프라이머 Forward에...

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WebA) The GreenGate cloning system uses six different types of pUC19 based entry vectors into which the individual elements are inserted and a pGreen-IIS based destination … WebFor this study, plasmid constructs were generated via GreenGate cloning . The protein-coding regions of MsSND1, ... For ectopic expression of transcription factors, the respective GreenGate construct was transformed into Agrobacterium tumefaciens ASE (pSOUP +). Selected transgenic clones were incubated in liquid LB medium containing the ...

WebDec 19, 2013 · A) The GreenGate cloning system uses six different types of pUC19 based entry vectors into which the individual elements are inserted and a pGreen-IIS based destination vector. Magenta scissors represent BsaI recognition sites. In each GreenGate reaction, six modules are ligated between the left border (LB) and the right border (RB) … WebDec 20, 2013 · GreenGate cloning is simple and efficient since it uses only one type IIS restriction endonuclease, depends on only six types of insert modules (plant promoter, N-terminal tag, coding sequence, C-terminal tag, plant terminator and plant resistance cassette), but at the same time allows assembling several expression cassettes in one …

WebGreenGate is a simple and efficient cloning system for rapidly assembling plant transformation constructs . It is based on the Golden Gate method. Using the type IIS … WebGreenGate cloning is simple and efficient since it uses only one type IIS restriction endonuclease, depends on only six types of insert modules (plant promoter, N-terminal tag, coding sequence, C ...

WebJan 20, 2024 · Here, we adapted the GreenGate cloning vectors to create two destination vectors showing strong marking of cell membranes in either the whole root or specifically in the lateral roots. This system can also be used in both embryos and whole seedlings. As proof of concept, we follow both gene expression and anatomy in Arabidopsis …

Web57 rows · GreenGate - A Novel, Versatile, and Efficient Cloning System for Plant Transgenesis. Lampropoulos A, Sutikovic Z, Wenzl C, Maegele I, Lohmann JU, Forner J … floor refinishing knoxville tnWebdestination vector for GreenGate cloning method, contains 2x a set of modules from A-F, "Driver line", tissues-specific promoter, Dex-inducible mTurquoise2 expression: greatpose.inWebJan 4, 2024 · promoter. Harnessing the flexibility of the GreenGate cloning system, we 57 produced a comprehensive set of GR-LhG4 driver lines targeting most tissues in the 58 . Arabidopsis. shoot and root with a strong focus on the indeterminate meristems. We show that, 59 when combined with effectors under control of the . pOp floor refinishing monmouth county njWebGreenGate cloning technology is sufficient to simultaneously construct a variety of effector expression vectors. All these make GreenGate versatile and applicable cloning system … greatposterwall authkeyWebJan 4, 2024 · Understanding the context-specific role of gene function is a key objective of modern biology. To this end, we generated a resource for inducible cell-type specific trans-activation based on the well-established combination of the chimeric GR-LhG4 transcription factor and the synthetic pOp promoter. Harnessing the flexibility of the GreenGate … great possibilities 意味WebGreenGate Cloning Kit Description: GreenGate is a simple and efficient cloning system for rapidly assembling plant transformation constructs. It is based on the Golden Gate … great posbrook farmWebGreenGate - A Novel, Versatile, and Efficient Cloning System for Plant Transgenesis. Lampropoulos A, Sutikovic Z, Wenzl C, Maegele I, Lohmann JU, Forner J PLoS One. 2013 Dec 20;8(12):e83043. doi: 10.1371/journal.pone.0083043. PubMed Plasmids from Article ... floor refinishing nashville