Cytochalasin b glucose uptake

Author: tvpj

August undefined, 2024

WebFeb 1, 2013 · RBCs from Atlantic salmon showed a linear relationship between glucose uptake and extracellular glucose level, but exhibited clear inhibition of glucose metabolism by cytochalasin B and phloretin, suggesting a component of facilitated glucose transport that is more elusive to detect. WebCytochalasin B (17·3 μ M) virtually abolished 3- O -methyl- D - [U- 14 C]glucose uptake and D - [5- 3 H]glucose utilization in tumoral insulin-producing cells of the RINm5F line. …

Discovery of a specific inhibitor of human GLUT5 by …

WebEnter the email address you signed up with and we'll email you a reset link. react tdk

The protein family of glucose transport facilitators: It

WebThis dependence results in up-regulation of glucose transporter expression, especially hGLUT1. The bound inhibitors include the natural compound cytochalasin B and two of a series of previously undescribed organic compounds that bind in the submicromolar range. WebJul 1, 1974 · Cytochalasin B in this concentration suppresses multiplication rates in a nutrient medium consisting of 2 % proteose peptone, but multiplication is unaffected if … WebA mold metabolite, cytochalasin B, inhibits uptake of 2-deoxy- d -glucose in chick fibroblasts and cultured liver cells. This inhibition is rapid, reversible, and is achieved at … how to stitch a quilt by hand

Cytochalasin B: Aspects of Phagocytosis in Nutrient …

Cryo-EM structure of human glucose transporter GLUT4

WebNov 10, 2014 · To further confirm our findings in primary brown adipocyte cultures, glucose uptake in BAT was measured in vivo in β 1 /β 2 knockout (to exclude any effects on β 1 - or β 2-adrenoceptors) and wild-type FVB mice that express all three β-adrenoceptor subtypes (Fig. 4, A and B). Isoproterenol increased glucose uptake in BAT in vivo in FVB and ... WebMay 13, 2024 · Insulin stimulates glucose uptake by acutely upregulating GLUT4 surface levels mainly through increased exocytosis of GSVs and decreased endocytosis 33, 34. A single GLUT4 molecule during its... how to stitch a shirt for menWebMay 9, 2024 · We artificially blocked the RBC GLUT1 transporter using either one of two routinely used GLUT1 inhibitors: cytochalasin B (CCB) and forskolin. These inhibitors bind GLUT1 in the transmembrane region, effectively blocking RBC D-glucose uptake (Guizouarn and Allegrini 2024 ). react tdah

"WebRCME cells exhibited stereospecific hexose uptake inhibited by cytochalasin B. Pretreatment of the RCME cells with potassium cyanide or 2,4-dinitrophenol inhibited 2-deoxyglucose uptake but not 3-O-methylglucose transport. A major proportion (30–60%) of the 2-deoxyglucose present in the RCME cells was not phosphorylated. " - Cytochalasin b glucose uptake

Cytochalasin b glucose uptake

Visfatin: A new player in mesangial cell physiology and diabetic ...

WebNov 2, 2011 · Breast cancer cells have a high level of glucose uptake and metabolism, a common characteristic of most cancer cells. This increased glucose uptake and aerobic metabolism forms the basis for assessment of tumor metabolism and response to therapy by positron emission tomography (PET) imaging with FDG (F-18 2-fluoro-2′-deoxyglucose) … Web2.2 Assaying Insulin-Stimulated Glucose Uptake 1. Serum-free media: DMEM (with 4.5 g/L glucose, l -glutamine, and sodium pyruvate) containing 0.5 % bovine serum albumin (BSA). 2. 2-Deoxyglucose (Sigma) stock: 100 m M (16.4 mg/mL in double-distilled water). Store at 2°–4°C up to 2 wk. 3.

Did you know?

WebMay 13, 2024 · Impairment of cellular glucose uptake due to compromised insulin ... we report the near atomic resolution cryo-EM structures of GLUT4 bound to a small … WebCytochalasin B (1 mg; Sigma-Aldrich; C6762). Add 2 ml DMSO into a vial with 1 mg cytochalasin B (500 μg; 100× CB stock solution). Divide into small tubes (10–20 μl) and store at − 80 °C. • TSA (1 mg; Sigma-Aldrich; T8552).

WebMar 5, 2010 · When expressed in S. cerevisae, rat GLUT1 showed a Km for D -glucose of 3.4 mM and transport was inhibited by cytochalasin B (IC50 = 0.44 μM), HgCl 2 (IC50 = 3.5 μM), phloretin (IC50 = 49 μM), and … WebCytochalasin B (17·3 μ M) virtually abolished 3- O -methyl- D - [U- 14 C]glucose uptake and D - [5- 3 H]glucose utilization in tumoral insulin-producing cells of the RINm5F line. This coincided with a marked decrease in D - [U- 14 C] glucose oxidation and suppression of the stimulant action of D -glucose upon insulin release.

WebA novel phosphoinositolglycan-peptide (PIG-P) from the yeast Saccharomyces cerevisiae potently mimicks insulin action on glucose transport and metabolism in rat muscle and adipose tissue. The aim of the present study was to elucidate the cellular WebApr 7, 2024 · It might be related to the F-actin disruption caused by ES, since the distribution of GLUT1 on cell surface could be changed from clusters to dispersed states when the actin cytoskeleton depolymerized by cytochalasin B. We further examined the glucose uptake capacity of cells after the ES using fluorescence glucalogue (2-NBDG), which has the ...

WebIn erythrocytes, adipocytes, and chick embryo fibroblasts, the Ki for glucose uptake inhibition and the Kd for cytochalasin B binding range from 0.1 to 0.4 µM. Values for hepatocytes and liver membranes are an order of magnitude higher …

Web2.2 Assaying Insulin-Stimulated Glucose Uptake. 1. Serum-free media: DMEM (with 4.5 g/L glucose, l-glutamine, and sodium pyruvate) containing 0.5 % bovine serum albumin … how to stitch an anarkali dressWebHepG2 cells treated with agents affecting glucose uptake/metabolism (phloretin, quercetin, quercetin-3′-sulfate, NaF, 3-bromopyruvate, NaN3, oligomycin A, ochratoxin A, cytochalasin B, and anti-GLUT1 antibody) showed dose-dependent changes in glucose and ATP levels without total protein (cell) loss. Finally, we performed flow cytometric ... how to stitch a stuffed animalWebApr 23, 2015 · Moreover, cytochalasin B, a nonspecific glucose transport inhibitor, also strongly inhibited the uptake of 14 C-glucose, 14 C-2DG and 2DG-IR in neuronal and astrocytic cultures . how to stitch anarkali dress at homeWebMar 1, 1990 · Glucose uptake in muscle occurs by a system of facilitated diffusion involving at least two distinct glucose transporters, GLUT-1 and GLUT-4. Both bind the fungal … react team ipswich hospitalWebMay 1, 1972 · Abstract. A mold metabolite, cytochalasin B, inhibits uptake of 2-deoxy-d-glucose in chick fibroblasts and cultured liver cells. This inhibition is rapid, reversible, … how to stitch a wound on a dogWebMay 13, 2024 · A bioluminescent glucose-uptake probe enables accurate, real-time, non-invasive longitudinal imaging of d-glucose absorption both in vitro and in vivo. ... To examine the effect of cytochalasin B ... how to stitch a wound shutWebIn vitro and in vivo study of phloretin-induced apoptosis in human liver cancer cells involving inhibition of type II glucose transporter. Chih Hsiung Wu, Yuan Soon Ho, Chia Yi Tsai, Ying Jan Wang, How Tseng, Po Li Wei, Chia Hwa Lee, Ren Shyan Liu, Shyr Yi Lin. react team sirona